By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)
Animal cellphone expertise is a becoming self-discipline of mobilephone biology which goals not just to appreciate buildings, services and behaviors of differentiated animal cells, but additionally to check their skills for use for commercial and scientific reasons. The aim of animal mobile know-how contains the clonal enlargement of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to provide proteins of clinical and pharmaceutical importantance, and the appliance of animal cells to gene treatment, synthetic organs and the construction of useful meals. This quantity offers the readers an entire evaluation of the current state of the art and should be precious for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, relatively mobilephone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines concerning animal cellphone culture.
Read Online or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 PDF
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Extra resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006
Example text
2E3-O cells were seeded in ASF104 medium with reduced growth supplements, and cultured in 96-well plates. 1% sericin (closed bar) or not (open bar). Caspase3/7 activity was measured 12 h after sericin treatment Table 2 Up- or down-regulated genes in sericin treatment Function Gene Proliferation and growth suppression cyclinD2 DDA3 HCK Cell survival and apoptosis fatso InSrr ASK1 caspase7 36 K. Yanagihara et al. References 1. S. Terada, T. Nishimura, M. Sasaki, H. Yamada, M. Miki (2002) Cytotechnology, 40, 3–12.
To reveal the mechanism, proteome analysis using two-dimensional electrophoresis (2-DE) was performed, where proteins were separated by isoelectric point in the first dimension using immobilized pH gradient gels, and subsequently by their molecular masses in the second dimension. Using this approach, proteins can be comprehensively studied. 1, by electric fusion with mouse spleen cells [2]. The cells were cultured in serum-free ASF103 or serum- and BSA-free ASF104 (Ajinomoto, Japan) at 37°C in humidified air containing 5% CO2.
Cytotechnolgy 28: 91–99. 4. Francis, G. , Ballard, F. , Milner, S. , McNeil, K. , Wallace, J. , King, R. and Wells, J. R. (1992) Novel Recombinant Fusion Protein Analogues of Insulin-Like Growth Factor (IGF)-I Indicate the Relative Importance of IGF-Binding Protein and Receptor Binding for Enhanced Biological Potency. Journal of Molecular Endocrinology 8 (3): 213–223. 5. , Negrete-Virgen, J. , Lyddiatt, A. and Al-Rubeai, M. (2002) Rapid Titration of Adenoviral Infectivity by Flow Cytometry in Batch Culture of Infected HEK 293 Cells.
