By Rajni Hatti-Kaul (auth.), Rajni Hatti-Kaul (eds.)
In Aqueous Two-Phase structures: equipment and Protocols, Rajni Hatti-Kaul and her specialist coauthors mix conception, method, and functions in a pragmatic number of simply reproducible protocols for bioseparations in aqueous two-phase platforms (ATPS). The protocols diversity from verified easy methods to state of the art innovations with capability biotechnological functions, all provided in set-by-step element to make sure effortless reproducibility and strong effects. one of the equipment designated are these for ATPS practise and characterization, for partitioning utilized to soluble molecules and particulates (including entire cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale dealing with of two-phase separations. suggestions for in situ product restoration in the course of biocatalytic techniques and for polymer-polymer structures in natural solvents also are provided.
useful and informative, with its precise directions permitting researchers to conform particular platforms to their very own separation wishes, Aqueous Two-Phase structures: equipment and Protocols demonstrates the scope and application of two-phase aqueous platforms in either easy and utilized research.
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Additional info for Aqueous Two-Phase Systems: Methods and Protocols: Methods and Protocols
The general procedures for performing microelectrophoresis are described by Seaman (8). The phases should be equilibrated at the temperature of the measurement, preferably in the same bath used to thermostat the apparatus. Suspensions of drops of one phase in the other are made by adding 5–20 μL of one phase to 5 mL of the other and agitating gently. The chamber is filled with the suspension and allowed to come to thermal equilibrium. The drops should be clearly seen with the optics focused at the stationary level in the chamber and should be moving only in the vertical plane with no lateral motion when the electric field is turned off.
87, 1883–1898. 8. Seaman, G. V. F. (1975) Electrokinetic behavior of red cells, in The Red Blood Cell, vol. 2 (Surgenor, D. ), Academic Press, NY, pp. 1135–1229. 9. Van Wazer, J. , Lyons, J. , Kim, K. , and Colwell, R. E. (1963) Viscosity and Flow Measurements. Interscience, NY, pp. 187–230. II PARTITIONING TECHNIQUES Single-Step Partitioning in ATPS 47 5 Single-Step Partitioning in Aqueous Two-Phase Systems Telma Teixeira Franco and Rajni Hatti-Kaul 1. g. proteins, nucleic acids, cells, viruses, organelles, and membranes.
For most applications the polymers are used as received (see Note 2). Polymer batches of narrow molecular weight distributions can also be purchased but at a much higher price (see Note 3). For example, less polydisperse Dx can be bought from Amersham Pharmacia Biotech (Uppsala, Sweden), whereas narrow fractions of PEG can be purchased from PolySciences (Warrington, PA). 2. Salts: Analytical grade salts can be purchased from any specialty chemicals manufacturer. 3. Buffers: A variety of buffers have been used to regulate the pH in aqueous twophase systems.